Long noncoding RNA TNFRSF10A-AS1 promotes colorectal cancer through upregulation of HuR

Figure 1 TNFRSF10A-AS1 is upregulated in colorectal cancer. A: TNFRSF10A-AS1 was upregulated in tumor tissues compared to normal tissues in TCGA colorectal cancer (CRC) database; B: TNFRSF10A-AS1 was overexpressed in CRC tumor tissues compared to matched adjacent nontumor tissues (n = 40); C: Levels of TNFRSF10A-AS1 in CRC were measured by reverse transcription-polymerase chain reaction (RT-PCR); D: TNFRSF10A-AS1 was higher in colon cancer cell lines than in colon mucosal epithelial FHC cells; E: The expression levels of TNFRSF10A-AS1 in the cytoplasm and nucleus of DLD1 and HCT116 cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). U2 snRNA was used as the internal reference for genes expressed in the nucleus, and S14 was used as the internal reference for genes expressed in the cytoplasm. COAD: Colon adenocarcinoma; READ: Rectum adenocarcinoma.