Identification of functional tumor necrosis factor-alpha promoter variants associated with Helicobacter pylori infection in the Sudanese population: Computational approach

Figure 6 Molecular detection of the tumor necrosis factor-alpha-1030 C/T polymorphism. A: PCR with confronting two-pair primer products analyzed on a 1% agarose gel stained with ethidium bromide. Three genotypes can be seen. Lanes 2, 3 and 5 showed 444 bp, 316 bp and 174 bp, indicating a heterozygous genotype. Lanes 4 and 6 showed 444 bp and 316 bp, which indicated a homozygous T genotype. Lane 1 showed 444 bp and 174 bp, which indicated a homozygous C genotype; B: Mammalian conservation of (TC; dbSNP: rs1799964) position -1030 among different species. MW: Molecular weight marker; C-ve: Negative control.