Ultrastructural changes in porcine liver sinusoidal endothelial cells of machine perfused liver donated after cardiac death

Figure 5 The ultrastructural characteristics in porcine liver sinusoidal endothelial cells preserved by midthermic machine perfusion. A: The distribution of ERG-positive liver sinusoidal endothelial cells (LSEC) in the porcine liver preserved by midthermic machine perfusion (MMP) for 4 h after 60 min of warm ischemia. Bar = 10 μm; B: Transmission electron microscopy image of the LSEC in porcine liver preserved by MMP for 4 hr after 60 min of warm ischemia. Bars = 1 μm; C and D: LSEC was observed by scanning electron microscopy in osmium-macerated porcine liver preserved by MMP for 4 hr after 60 min of warm ischemia. s: Sinusoid. h: Hepatocyte. The space of Disse was indicated by an asterisk. The partial area indicated in C was further observed in high magnification D. Bars = 1 μm. Arrows indicate the rER. Mitochondria are labeled in green; the nucleus is labeled in blue, expanded rER is labeled in red. TEM: Transmission electron microscopy; SEM: Scanning electron microscopy.