Ultrastructural changes in porcine liver sinusoidal endothelial cells of machine perfused liver donated after cardiac death

Figure 4 The ultrastructural alteration in porcine liver sinusoidal endothelial cells preserved by hypothermic machine perfusion. A: The distribution of viable liver sinusoidal endothelial cells (LSEC) which is indicated with ERG-positive cells in the porcine liver preserved by hypothermic machine perfusion (HMP) for 4 h after 60 min of warm ischemia. Bar = 10 μm; B: transmission electron microscopy image of the LSEC in porcine liver preserved by HMP for 4 h after 60 min of warm ischemia. Bars = 1 μm; C and D: LSEC was observed by scanning electron microscopy in osmium-macerated porcine liver preserved by HMP for 4 h after 60 min of warm ischemia. s: Sinusoid. h: Hepatocyte. The partial area indicated in C was further observed in high magnification D. Bars = 1 μm. Mitochondria are labeled in green and the nucleus is labeled in blue. Arrows indicate the rER. TEM: Transmission electron microscopy; SEM: Scanning electron microscopy.