Ultrastructural changes in porcine liver sinusoidal endothelial cells of machine perfused liver donated after cardiac death

Figure 3 Changes in the intracellular ultrastructure of the porcine liver sinusoidal endothelial cells after warm ischemia. A: The distribution of viable liver sinusoidal endothelial cells (LSEC) indicated by ERG-positive cells in the porcine liver after warm ischemia for 60 min. Bar = 10 μm; B: Transmission electron microscopy image of the LSEC after warm ischemia. Bars = 1 μm; C and D: LSEC after warm ischemia was observed by SEM in the osmium-macerated porcine liver. s: sinusoid. h: Hepatocyte. The space of Disse was indicated by an asterisk. The partial area indicated in C was further observed in high magnification D. Bars = 1 μm. Mitochondria is represented in green and the nucleus is represented in blue. Arrows indicate the rER. The rER surround the mitochondria were indicated by arrowheads. TEM: Transmission electron microscopy; SEM: Scanning electron microscopy.