Sirtuin1 attenuates acute liver failure by reducing reactive oxygen species via hypoxia inducible factor 1α

Figure 2 Hypoxia decreased Sirtuin1 expression leading to the acetylation and activation of hypoxia inducible factor-1α. A-D: Western blotting was performed to measure the levels of Sirtuin1 (Sirt1), Bcl-2 adenovirus E1B-interacting protein 3 (Bnip3), and hypoxia inducible factor (HIF)-1α in L02 cells; E: The representative images of immunofluorescence staining for Acetyl-lysine and HIF-1α; F and G: Equal amounts of protein were subjected to immunoprecipitation with Sirt1 antibody or HIF-1α antibody followed by immunoblotting with antibody against Sirt1, HIF-1α, or acetyl-lysine and effect of Sirt1 overexpression (O/E) was shown. Data shown are means ± standard deviations (SDs) of three separate experiments. ^aP < 0.05 vs Control group; one-way analysis of variance combined with Bonferroni's post hoc test; the error bars indicate the SDs.