Sirtuin1 attenuates acute liver failure by reducing reactive oxygen species via hypoxia inducible factor 1α

Figure 1 Hypoxia aggravated acute liver failure and increased the expression of hypoxia inducible factor-1α and its acetylation. A: The representative images of hematoxylin and eosin staining of liver in each group; B: Western blotting was performed to measure the levels of Sirtuin1 (Sirt1), Bcl-2 adenovirus E1B-interacting protein 3 (Bnip3) and carbonic anhydrase 9 (CA9) in liver tissues; C: The representative images of immunofluorescence staining for Acetyl-lysine and hypoxia inducible factor (HIF)-1α. Data shown are means ± standard deviation of three separate experiments. ^aP < 0.05 vs Control group; ^bP < 0.05 vs Lipopolysaccharide (LPS)-treated group; one-way analysis of variance combined with Bonferroni's post hoc test; the error bars indicate the standard deviations. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.