LncRNA cancer susceptibility 20 regulates the metastasis of human gastric cancer cells via the miR-143-5p/MEMO1 molecular axis

Figure 6 Inhibition of miR-143-5p blocked the function of MEMO1 knockdown in gastric cancer cells. A-D: MGC803 and SGC7901 cells were transfected with si-NC, si-MEMO1, si-MEMO1 + inhibitor NC, si-MEMO1 + miR-143-5p inhibitor, respectively. Transwell assay was employed for the detection of cell invasion and migration ability; E-G: MGC803 and SGC7901 cells were transfected with si-NC, si-MEMO1 + inhibitor NC, si-NC + miR-143-5p inhibitor, si-MEMO1 + miR-143-5p inhibitor, respectively. Wound healing assay was used to detect migration ability; H: The protein expression of MEMO1 was detected by western blot assay after transfecting with miR-143-5p mimics or si-cancer susceptibility 20; I: Western blot was utilized to detect the expressions of EMT-related proteins (ZEB1, Vimentin and E-cadherin). ^aP < 0.05 vs control.