Establishing a rabbit model of perianal fistulizing Crohn’s disease

Figure 1 Photograph of the external opening of the anal fistula, which was approximately 1 cm away from the anus, and a flowchart of the study protocol. A: The rubber band was used to hang the seton, and the leather band was fixed with a No. 0 operation seton to prevent slippage; B: One rabbit died one week after surgery. The remaining 17 rabbits were marked according to the length of insertion time. Three rabbits each were randomly selected from groups A and B. TNBS: 2,4,6-Trinitrobenzene sulfonic acid; DAI: Disease activity index; EUS: Endoscopic ultrasonography.