Syngeneic implantation of mouse hepatic progenitor cell-derived three-dimensional liver tissue with dense collagen fibrils

Figure 4 Transplanted three-dimensional liver tissue culture models in partially hepatectomized model mice. A: A schematic illustration showing the transplantation of the three-dimensional (3-D) liver tissue culture model; B: Histological analyses and hematoxylin-eosin staining of the section for the 3-D liver tissue culture model after transplantation; C: Higher magnification of the inscribed area in (B). The vascularization was observable at condensed collagen fibril matrices. Arrowheads indicate new blood vessels; D: Immunohistochemical analysis of the 3-D liver tissue culture model of albumin-positive hepatic cells after being transplanted using anti-albumin (green) antibodies. Bar corresponds to 100 μm.