Syngeneic implantation of mouse hepatic progenitor cell-derived three-dimensional liver tissue with dense collagen fibrils

Figure 3 Validation of hepatic functions in the three-dimensional liver tissue culture model. A and B: Urea (A) and albumin (B) production levels in the culture medium in each culture condition; C: Hydroxylation activity of testosterone in the three-dimensional (3-D) liver tissue culture model. The amount of each hydroxylated testosterone in culture medium. Culture medium was changed every 2 d over the course of the measurement period. 2-D culture (open columns) and 3-D culture (closed columns) system. Statistical comparisons were made using the Welch t-test. Data are shown as means ± SE, n = 3, ^aP < 0.01.