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©The Author(s) 2022.
World J Gastroenterol. Mar 14, 2022; 28(10): 1024-1054
Published online Mar 14, 2022. doi: 10.3748/wjg.v28.i10.1024
Published online Mar 14, 2022. doi: 10.3748/wjg.v28.i10.1024
Figure 3 The trypsin activation observed in LAT1-ko mice had higher variability, and the expression of trypsinogens and cathepsins differed from LAT1-wt mice.
A: Trypsin activation was observed in LAT1-wt and LAT1-ko mice 24 h after acute pancreatitis (AP) induction. The activity was measured using Boc-Q-A-R-MCA and expressed as nmol/μg DNA. Mean ± SEM, n = 3-7; B-C: The mRNA expression of trypsinogens, trypsin inhibitor, and cathepsins. The mRNA expressions of serine protease 1+3 (prss1+3) and serine protease 2 (prss2) along with serine peptidase inhibitor kazal type 1 (spink1) (B) and cathepsin B, D, and L (ctsb, ctsd, and ctsl) (C) were analyzed via qPCR and expressed relative to a housekeeping gene (18S × 106) in LAT1-wt and LAT1-ko mice. The upper panel depicts the mice that were injected with saline, and the lower panel shows the mice 24 h after AP induction. Mean ± SEM, n = 7-14. Statistical analysis was conducted with ANOVA and Bonferroni and compared, as indicated by the capped line. aP < 0.05, bP < 0.01, cP < 0.001.
- Citation: Hagen CM, Roth E, Graf TR, Verrey F, Graf R, Gupta A, Pellegrini G, Poncet N, Camargo SMR. Loss of LAT1 sex-dependently delays recovery after caerulein-induced acute pancreatitis. World J Gastroenterol 2022; 28(10): 1024-1054
- URL: https://www.wjgnet.com/1007-9327/full/v28/i10/1024.htm
- DOI: https://dx.doi.org/10.3748/wjg.v28.i10.1024