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©The Author(s) 2022.
World J Gastroenterol. Mar 14, 2022; 28(10): 1024-1054
Published online Mar 14, 2022. doi: 10.3748/wjg.v28.i10.1024
Published online Mar 14, 2022. doi: 10.3748/wjg.v28.i10.1024
Table 2 Antibodies and conditions applied in Western blotting experiments
Antigen | Total protein loaded (µg) | Primary antibody | Antibody dilution |
Amylase | 2.5 | LifeSpan BioSciences, Inc. (LS-B8239) | 1:10000 |
β-tubulin | Varying | Sigma (T8328) | 1:5000 |
LAT1 | 20 | Cosmobio (KE026) | 1:500 |
LC3 | 100 | MLB (PM036) | 1:250 |
NA/K-ATPase | Varying | Santa Cruz (sc-48345) | 1:10000 |
p62 | 50 | MBL (PM045) | 1:1000 |
peIF2α | 25 | Cell Signaling (3398) | 1:1000 |
pS6K | 50 | Cell Signaling (9205S) | 1:1000 |
SMA | 25 | Sigma (C6198) | 1:1000 |
teIF2α | 25 | Cell Signaling (9722) | 1:1000 |
tS6K | 50 | Cell Signaling (2708P) | 1:1000 |
Vimentin | 30 | Invitrogen (MA5-11883) | 1:1000 |
- Citation: Hagen CM, Roth E, Graf TR, Verrey F, Graf R, Gupta A, Pellegrini G, Poncet N, Camargo SMR. Loss of LAT1 sex-dependently delays recovery after caerulein-induced acute pancreatitis. World J Gastroenterol 2022; 28(10): 1024-1054
- URL: https://www.wjgnet.com/1007-9327/full/v28/i10/1024.htm
- DOI: https://dx.doi.org/10.3748/wjg.v28.i10.1024