Abdominal paracentesis drainage attenuates intestinal inflammation in rats with severe acute pancreatitis by inhibiting the HMGB1-mediated TLR4 signaling pathway

Figure 5 Effects of pancreatitis-associated ascitic fluid intraperitoneal injection with or without anti-high mobility group box protein 1 neutralizing antibody on intestinal toll-like receptor 4 signaling pathway in cerulein-treated rats. A: High mobility group box protein 1 in serum; B: Toll-like receptor 4 (TLR4) mRNA measurement by real-time polymerase chain reaction; C: Immunoblotting of TLR4, myeloid differentiation factor 88 (MyD88) and p65 protein expression and p65 phosphorylation level from intestine samples; D: Representative immunohistochemical images of TLR4 in intestinal tissue (bar = 100 μm); E-G: Densitometry analysis of TLR4, MyD88 and ratio of p65 to phosphorylated p65. Data are expressed as means ± SD, n = 6 enzyme-linked immunosorbent assay results per group; means ± SD, n = 3 immunoblotting results per group. ^aP < 0.05 vs control group; ^bP < 0.05 vs pancreatitis-associated ascitic fluid injection group. HMGB1: High mobility group box protein 1; CN: Control; PI: Pancreatitis-associated ascitic fluid (PAAF) injection; PIH: PAAF and 200 μg anti-HMGB1 neutralizing antibody; PIC: PAAF + control lgY; TLR4: Toll-like receptor 4; MyD88: Myeloid differentiation factor 88; p-p65: Phosphorylated p65; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.