Circular RNA circVAPA promotes chemotherapy drug resistance in gastric cancer progression by regulating miR-125b-5p/STAT3 axis

Figure 6 CircVAPA promotes chemotherapy drug resistance of gastric cancer cells by modulating miR-125b-5p/STAT3 axis. A and B: SGC7901 and HGC27 cells were treated with cisplatin (DDP), DDP, and circVAPA shRNA, or co-treated with DDP, circVAPA shRNA, and miR-125b-5p inhibitor. Cell viability was measured by MTT assay; C: SGC7901/DDP cells were treated with DDP or co-treated with DDP and pcDNA3.1-circVAPA at the indicated dose, or co-treated with DDP, pcDNA3.1-circVAPA, and STAT3 siRNA. Cell viability was measured by MTT assay; D and E: SGC7901 and HGC27 cells were treated with DDP, DDP, and circVAPA shRNA, or co-treated with DDP, circVAPA shRNA, and miR-125b-5p inhibitor or pcDNA3.1-STAT3. Cell apoptosis was analyzed by flow cytometry. Data are presented as the mean ± SD. ^bP < 0.01. DDP: Cisplatin.