Circular RNA circVAPA promotes chemotherapy drug resistance in gastric cancer progression by regulating miR-125b-5p/STAT3 axis

Figure 4 CircVAPA is able to sponge miR-125b-5p in gastric cancer cells. A: The interaction of CircVAPA and miR-125b-5p was analyzed by bioinformatic analysis based on ENCORI (http://starbase.sysu.edu.cn/index.php); B-D: SGC7901 and HGC27 cells were treated with control mimic or miR-125b-5p mimic. The expression levels of miR-125b-5p were tested by qPCR in the cells (B), and luciferase activities of wild type CircVAPA and CircVAPA with the miR-125b-5p-binding site mutant were examined by luciferase reporter gene assay (C and D); E and F: SGC7901 and HGC27 cells were treated with the circVAPA shRNA or control shRNA. The expression of miR-125b-5p was analyzed by qPCR assay. Data are presented as the mean ± SD. ^bP < 0.01. MUT: Mutant; WT: Wild type.