Hepatitis B core antigen modulates exosomal miR-135a to target vesicle-associated membrane protein 2 promoting chemoresistance in hepatocellular carcinoma

Figure 4 Vesicle-associated membrane protein 2 contributed to anti-apoptosis and proliferation induced by miR-135a-5p. A and B: Cell apoptosis was examined by flow cytometry in HepG2 cells transfected with the specific plasmid combinations; C: B-cell lymphoma-2 expression was detected by Western blot in HepG2 cells after transfection with the indicated plasmids; D: Cell counting kit 8 assay showed the proliferation of HepG2 cells after transfection with the plasmid combination shown above; E: The protein level of mini-chromosome maintenance protein-2 and proliferating cell nuclear antigen was measured by Western blot in HepG2 cells transfected with the plasmid group shown in the figure above. ^aP < 0.05; ^bP < 0.01. Bcl-2: B-cell lymphoma-2; MCM2: Mini-chromosome maintenance protein-2; PCNA: Proliferating cell nuclear antigen; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; VAMP2: Vesicle-associated membrane protein 2.