Hepatitis B core antigen modulates exosomal miR-135a to target vesicle-associated membrane protein 2 promoting chemoresistance in hepatocellular carcinoma

doi:10.3748/wjg.v27.i48.8302

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Dec 28, 2021 (publication date) through Sep 8, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 28, 2021; 27(48): 8302-8322
Published online Dec 28, 2021. doi: 10.3748/wjg.v27.i48.8302

Table 2 Sequences and primers for vector construction

Category	Sequence (5’-3’)
miR-135a-5p mimic	F-UAUGGCUUUUUAUUCCUAUGUGA
miR-135a-5p mimic	R-UCACAUAGGAAUAAAAAGCCAUA
miR-135a-5p inhibitor	UCACAUAGGAAUAAAAAGCCAUA
VAMP2 (NM_001330125.1)	F-CTAGCTAGCATGGACAGGTCTGCTAC
VAMP2 (NM_001330125.1)	R-CGCGGATCCTTAAGTGCTGAAGT
VAMP2 3’-UTR-WT	F-CTAGCTAGCATCCCCGAGGAGTCT
VAMP2 3’-UTR-WT	R-ACGCGTCGACAGAGAGGGGTGAAG
VAMP2 3’-UTR-MUT	F-GTTCCTCCACCTCTCACGTGCATCTTTCAGCC CC
VAMP2 3’-UTR-MUT	R-GGGGCTGAAAGATGCACGTGAGAGGTGGAGGAAC
Hepatitis B virus-1903/2454	F-CTAGCTAGCGCCACCATGGACATCGACCCTT
Hepatitis B virus-1903/2454	R-CCGCTCGAGCTAACATTGAGATTCCCGAGAT

VAMP2: Vesicle-associated membrane protein 2; WT: Wild-type; MUT: Mutant.

Citation: Wei XC, Xia YR, Zhou P, Xue X, Ding S, Liu LJ, Zhu F. Hepatitis B core antigen modulates exosomal miR-135a to target vesicle-associated membrane protein 2 promoting chemoresistance in hepatocellular carcinoma. World J Gastroenterol 2021; 27(48): 8302-8322
URL: https://www.wjgnet.com/1007-9327/full/v27/i48/8302.htm
DOI: https://dx.doi.org/10.3748/wjg.v27.i48.8302