Recombinant protein Schistosoma japonicum-derived molecule attenuates dextran sulfate sodium-induced colitis by inhibiting miRNA-217-5p to alleviate apoptosis

Figure 4 rSj16 have anti-apoptotic action by regulating the miR-217-5p/HNF1B axis. A and B: MODE-K cells were treated with Etoposide, Etoposide + miR-217-5p, and Etoposide + miR-217-5p + rSj16. The expression of apoptosis relative proteins, including Bcl-2, Bax and cleaved-Caspase3 was analyzed by Western blotting; C and D: The apoptosis of MODE-K cells was detected by TUNEL assay after treatment with Etoposide + miR-217-5p, and Etoposide + miR-217-5p + rSj16 (10×), TUNEL positive cells were apoptotic cells, the number of TUNEL positive cells was quantified; E and F: Flow cytometry analysis of MODE-K cells treated with Etoposide + miR-217-5p, and Etoposide + miR-217-5p + rSj16. Statistical analysis was performed using one-way ANOVA. PI: propidium iodide. Data are presented as means ± SD; ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.