Recombinant protein Schistosoma japonicum-derived molecule attenuates dextran sulfate sodium-induced colitis by inhibiting miRNA-217-5p to alleviate apoptosis

Figure 2 rSj16 inhibits dextran sulfate sodium induced apoptosis of colon epithelial cells. A: Western blot analysis for the expression of apoptosis relative proteins, including Bcl-2, Bax and cleaved-Caspase3; B: The apoptosis of colon tissue of mice treated with dextran sulfate sodium (DSS) + PBS and DSS + rSj16 was detected by TUNEL assay (20×), TUNEL positive cells were apoptotic cells, the number of TUNEL positive cells was quantified. TUNEL: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Statistical analysis was performed using one-way ANOVA (A) and Kruskal–Wallis test (non-parametric) (B). Data are presented as means ± SD; ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.