Enteric nervous system and inflammatory bowel diseases: Correlated impacts and therapeutic approaches through the P2X7 receptor

Table 2 Specific variations in cell number and neuronal profile area according to the respective chemical code observed in the myenteric plexus of the enteric nervous system

Ref.	Species	Colitis	Time	Myenteric plexus
				Cellular chemical code (change in density compared to healthy tissues, %)	Cellular chemical code (change in profile area compared to healthy tissues, %)
Boyer et al[79]	Mice	DNBS-50% ethanol	0.5 - 120 h	HuC/D (< 42%-CT)	N/A
Linden et al[17]	Guinea pig	TNBS-30% ethanol	2 - 12 h;1-56 d	HuC/D 12 and 24 h (< 15%-CT); HuC/D 6 and 56 d (< 20%-CT); ChAT, nNOS, calret and NeuN 6 d (=); VIP 6 (>)1 and 56 d (No differences)	N/A
Sarnelli et al[171]	Rat	TNBS-50% ethanol	7 d	HuC/D (< 20%-CT)	N/A
Gulbransen et al[19]	Mice	DNBS-50% ethanol	48 h	HuC/D (< 32%-CT)	N/A
Linden[77]	Guinea pig	TNBS-30% ethanol	24 h	HuC/D (< approximately 20%-25%-CT)	N/A
Da Silva et al[129]	Rat	TNBS-30% ethanol	24 h	P2X7 (< 11%-CT); ChAT (< 34.9%-CT); nNOS (< 42.3%-CT; < 18%-sham); Calret (< 60.6%-CT; < 15%-sham); Calbindin (< 22.9%-CT); HuC/D (< 33.3%-CT; < 16%-sham); S100β (< 29.2%-CT; < 23%-sham)	nNOS (< 6.6%-CT/sham); ChAT (< 21.2%-CT/sham); Calbindin (>19%-CT); Calretinin (< 2%-sham)
Souza et al[133],2	Rat	TNBS-30% ethanol	24 h	P2X7 (< 10.6%-sham; < 20.4%-BBG); ChAT (< 34%-sham; < 13.9%-BBG); nNOS (< 22.9%-sham; < 22.2%-BBG); HuC/D (< 15.4%-sham; < 19.5%-BBG); GFAP (< 14.4%-sham; < 17.7%-BBG)	nNOS (< 12%-sham; < 8%-BBG); ChAT and HuC/D (No differences)

¹Change in count without percentage information.

²Data from ileum after colitis. DNBS: Dinitrobenzene sulfonic acid; TNBS: 2,4,6-trinitrobenzenesulfonic acid; N/A: Not applicable; (=): Similarity of cell count; (<): Cell count/area decrease; (>): Cell count/area increase; ir: Immunoreactive; CT: Control group; Sham: Sham group; BBG: Brilliant Blue G-treated animals group; P2X7: P2X7 receptor; ChAT: Acetylcholine transferase enzyme-ir; nNOS: Neuronal nitric oxide synthase enzyme-ir; Calret: Calretinin-ir; VIP: Vasoactive intestinal polypeptide-ir; HuC/D: Neuronal pan-ir; NeuN: Neuronal nuclear antigen-ir; GFAP: Glial fibrillary acid protein-ir; S100β: Protein β for calcium S100-ir labeling.