Autosomal recessive 333 base pair interleukin 10 receptor alpha subunit deletion in very early-onset inflammatory bowel disease

Figure 3 Histopathochemistry and functional results of homozygous interleukin 10 receptor alpha subunit mutation. A: Histological findings in a colonic specimen obtained during colonoscopy showing oval-shaped intramural abscesses; B: Higher magnification of inset A; C: Determination of TNF-α levels in the supernatant of cultured PBMCs from patient D after stimulation with PBS, LPS, or LPS + IL-10 in vitro; D: Western blot results of PBMCs isolated from patient D after stimulation with LPS or IL-10. Phosphorylation of STAT3 at Tyr705 and Ser727 and total STAT3 protein were detected (n = 3). ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001 were considered as statistically different.