Liver injury changes the biological characters of serum small extracellular vesicles and reprograms hepatic macrophages in mice

Figure 1 Establishment and validation of CCL₄-induced acute liver injury and chronic liver injury in mice. A: Workflow for the establishment of the acute liver injury (ALI) and chronic liver injury (CLI) mouse models; B: Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in ALI mice. Compared with the ALI control (AC) group, ^aP < 0.05, ^bP < 0.01; C: Hematoxylin-eosin (H&E) and Sirius Red staining of liver sections from AC, 2 d and 7 d mice; D: Serum ALT and AST levels in CLI mice. Compared with the CC group, ^aP < 0.05; E: H&E and Sirius Red staining of liver sections from CLI control (CC), 8 wk and 12 wk mice. Scale bar = 100 μm. D: Day; W: Week.