Fusobacterium nucleatum colonization is associated with decreased survival of helicobacter pylori-positive gastric cancer patients

Figure 1 Changes in the gene expression profile of 008L-C2 cancer cells cocultured with Fusobacterium nucleatum. Fusobacterium nucleatum was added to the cell culture at multiplicity of infection (MOI) 10 and 100. After 24 h and 72 h of incubation, the cells were rinsed extensively to remove unattached bacteria. RNA was collected and analyzed using RNA sequencing. The cells collected at time 0 were used as control. Sequencing reads were trimmed and mapped to hg19 using CLC Genomic Workbench v.12.0.3. The colored dots (green for MOI = 10 and red for MOI = 100) are genes showing more than four-fold change at the respective incubation time. Genes with transcripts per million < 1 in all datasets and less than a four-fold change are not shown.