Cold exposure and capsaicin promote 1,2-dimethylhyrazine-induced colon carcinogenesis in rats correlates with extracellular matrix remodeling

Figure 7 Changes in matrix metalloproteinase 1, matrix metalloproteinase 2, matrix metalloproteinase 9, and tissue-specific matrix metalloproteinase 1 proteins in the colonic tissues of different treatment groups. A: Protein expressions of matrix metalloproteinase (MMP) 1, MMP2, MMP9 and tissue-specific matrix metalloproteinase 1 (TIMP1) in the colonic tissues via immunohistochemical staining. Magnification, × 400, scalar bar 20 μm; B-E: Densitometric analysis of MMP1 (B), MMP2 (C), MMP9 (D) and TIMP1 (E) during immunohistochemical staining. ^aP < 0.05, ^bP < 0.01, control compared with 1,2-dimethylhyrazine (DMH); ^cP < 0.05, ^dP < 0.01, DMH compared with cold exposure and capsaicin-treated group; ^eP < 0.05, cold exposure compared with capsaicin-treated group.