Cold exposure and capsaicin promote 1,2-dimethylhyrazine-induced colon carcinogenesis in rats correlates with extracellular matrix remodeling

Figure 6 Changes in collagen, elastin and lysyl oxidase-like-2 proteins in the colonic tissues of different treatment groups. A: Protein expressions of type I collagen (COL I), type III collagen (COL III), lysyl oxidase-like-2 (LOXL2), and elastin in the colonic tissues via immunohistochemical staining. Magnification, × 400, scalar bar 20 μm; B-I: Densitometric analysis of COL I (B), COL III (C), LOXL2 (D), elastin(E), COL I/COL III (F), COL I area (G), COL III area (H), and COL I area/COL III area (I) during immunohistochemical staining. ^aP < 0.05, ^bP < 0.01, control compared with 1,2-dimethylhyrazine (DMH); ^cP < 0.05, ^dP < 0.01, DMH compared with cold exposure and capsaicin-treated group; ^eP < 0.05, ^fP < 0.01, cold exposure compared with capsaicin-treated group.