Cold exposure and capsaicin promote 1,2-dimethylhyrazine-induced colon carcinogenesis in rats correlates with extracellular matrix remodeling

Figure 4 Collagen fibers were automatically extracted for analysis using open-source software CT-FIRE. A: Histograms were generated to show the distribution of various parameters in each polarized light microscopy imaging; B: Quantitative analysis of collagen fibers from polarized light microscopy imaging in the colonic mucosa of different treatment groups. Data are mean ± SE of three images per tissues region. ^aP < 0.05, ^bP < 0.01, control compared with 1,2-dimethylhyrazine (DMH); ^cP < 0.05, ^dP < 0.01, DMH compared with cold exposure and capsaicin-treated group; ^eP < 0.05, Cold exposure compared with capsaicin-treated group.