Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection

Figure 5 Immunohistochemical staining for 8-oxo-7,8-dihydro-2-deoxyguanosine in tissues. A: Representative images showing immunohistochemical staining (400 × magnification) of 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxo-dGsn; a DNA oxidative damage marker) in colon sections of rats. Left panel images (PBS) indicate the results of the control group. Middle panel images (D1) indicate the results one day after Shigella flexneri (S. flexneri) infection. Right panel images (D5) indicate the results five days after S. flexneri infection; B: Immunohistochemical staining for 8-oxo-dGsn in ileum sections; C: Immunohistochemical staining for 8-oxo-dGsn in liver sections; D: Immunohistochemical staining for 8-oxo-dGsn in spleen sections; E: Immunohistochemical staining for 8-oxo-dGsn in brain sections. Histogram: The quantitative analysis of immunohistochemical staining of 8-oxo-dGsn in tissues from the infection and control groups. Scale bar, 50 μm. ^aP < 0.05. IOD: Integrated optical density.