Hepatitis B virus persistent infection-related single nucleotide polymorphisms in HLA regions are associated with viral load in hepatoma families

Table 4 Factors associated with hepatitis B viral load in a hepatitis B surface antigen-positive hepatocellular carcinoma family cohort

	HBV DNA
Category	≥ 105 cps/mL	< 105 cps/mL	OR (95%CI)	Adjusted OR (95% CI)2		P value	Adjusted P value2
Total members	147	162
Age in yr, mean ± SD	45.03 ± 14.18	41.82 ± 14.21	1.017 (1-1.035)	1.017 (0.999-1.035)		0.0538	0.0668
Sex, n (%)
Male	100 (68.03)	88 (54.32)	1.922 (1.188-3.111)	1.914 (1.187-3.087)		0.0078	0.0078
Female	47 (31.97)	74 (45.68)	1	1
Relation to index, n (%)
Children and grandchildren generation	58 (39.46)	95 (58.64)	1	1
Parent generation	5 (3.4)	2 (1.23)	3.683 (0.866-15.656)	5.056 (1.259-20.3)		0.0775	0.0223
Index generation	84 (57.14)	65 (40.12)	2.033 (1.274-3.246)	1.845 (1.144-2.977)		0.0029	0.0121
Index’s status, n (%)
HBsAg-	21 (14.29)	49 (30.25)	1	1
HBsAg+	126 (85.71)	113 (69.75)	2.508 (1.351-4.657)	2.492 (1.324-4.692)		0.0036	0.0047
Mother’s status, n (%)
HBsAg-	43 (29.25)	43 (26.54)	1	1
HBsAg+	46 (31.29)	51 (31.48)	0.874 (0.467-1.634)	0.91 (0.485-1.707)		0.6724	0.7693
Unknown	58 (39.46)	68 (41.98)	0.855 (0.491-1.49)	0.857 (0.488-1.503)		0.5804	0.5898
HBV genotype (BGT230), n (%)
Unknown3	3 (2.05)	72 (44.44)	0.03 (0.009-0.104)	0.029 (0.008-0.1)		< 0.0001	< 0.0001
B	102 (69.86)	75 (46.3)	1	1
C	41 (28.08)	15 (9.26)	2.042 (1.023-4.079)	2.066 (0.995-4.288)		0.0431	0.0515
SGPT, mean ± SD	63.92 ± 79.63	37.02 ± 45.22	1.010 (1.002-1.018)	1.009 (1.001-1.017)		0.0105	0.0260
rs477515 (MAF = 0.1149) Chr6: 326019141
TT (reference)	1 (0.68)	4 (2.47)
TC	15 (10.2)	46 (28.4)
CC	131 (89.12)	112 (69.14)	3.107 (1.708-5.653)	3.195 (1.746-5.847)	0.0002	0.0002
rs9272105 (MAF = 0.4078) Chr6: 326322221
GG (reference)	20 (13.61)	36 (22.22)
GA	59 (40.14)	81 (50)
AA	68 (46.26)	45 (27.78)	1.747 (1.256-2.428)	1.75 (1.247-2.456)	0.0009	0.0012
rs9276370 (MAF = 0.07605) Chr6: 327395181
GG (reference)	1 (0.68)	3 (1.85)
GT	14 (9.52)	25 (15.43)
TT	132 (89.8)	134 (82.72)	1.747 (0.933-3.272)	1.679 (0.901-3.131)	0.0811	0.1029
rs7756516 (MAF = 0.08091) Chr6: 327561401
CC (reference)	0 (0)	4 (2.47)
CT	16 (10.88)	26 (16.05)
TT	131 (89.12)	132 (81.48)	1.951 (1.078-3.53)	1.875 (1.029-3.417)	0.0272	0.0400
rs9277535 (MAF = 0.2589) Chr6: 330870841
AA (reference)	13 (8.84)	8 (4.94)
AG	45 (30.61)	73 (45.06)
GG	89 (60.54)	81 (50)	1.235 (0.849-1.797)	1.303 (0.888-1.911)	0.2703	0.1767

¹Genome Build ARCH38.

²Adjusted by sex.

³Eight cases not tested. CI: Confidence interval; HBsAg: Hepatitis B surface antigen; OR: Odds ratio; HBV: Hepatitis B virus.