Hepatitis B virus persistent infection-related single nucleotide polymorphisms in HLA regions are associated with viral load in hepatoma families

Table 2 Cumulative effect of the genetic-risk alleles associated with hepatitis B viral load or persistent hepatitis B virus infection

Study	WGRS quartile	OR (95%CI)	P value
Family first stage: Persistent HBV infection	Q1 (WGRS ≤ 6.166)	1
	Q2 (WGRS = 6.166-7.083)	1.878 (1.277-2.762)	0.0014
	Q3,4 (WGRS > 7.083)1	2.538 (1.742-3.698)	< 0.0001
	Cochran-Armitage trend test		< 0.0001
Family second stage: Viral load	Q1 (WGRS ≤ 4.583)	1
	Q2 (WGRS = 4.583-5.291)	2.204 (1.253-3.878)	0.0061
	Q3,4 (WGRS > 5.291)2	3.156 (1.780-5.595)	< 0.0001
	Cochran-Armitage trend test		< 0.0001

¹The number of hepatitis B surface antigen negative individuals in Q4 was < 5, so Q3 and Q4 were combined.

²The number of individuals with hepatitis B virus DNA < 10⁵ cps/mL in Q4 was < 1, so Q3 and Q4 were combined. The cumulative effect was calculated from: Four single nucleotide polymorphisms (SNPs) (rs9272105, rs9276370, rs7756516, and s9277535) in unrelated male hepatitis B surface antigen (HBsAg) carriers; four SNPs (rs477515, rs9276370, rs7756516, and rs9277535) in the first-stage hepatocellular carcinoma (HCC) family cohort analysis; and three SNPs (rs477515, rs9272105, and rs7756516) in HBsAg-positive carriers in the second-stage HCC family cohort analysis. CI: Confidence interval; OR: Odds ratio; Q: Quartile; WGRS: Weighted genetic risk score; HBV: Hepatitis B virus.