Exosomal microRNA-588 from M2 polarized macrophages contributes to cisplatin resistance of gastric cancer cells

Figure 3 Exosomal miR-588 from M2 macrophages contributes to cisplatin resistance of gastric cancer cells. A: Detection of expression of miR-588 by qPCR in the exosomes from M2 macrophages and inactivated macrophages; B: Detection of expression of miR-588 by qPCR in cisplatin (DDP)-resistant SGC7901 cells, M2 macrophages, and inactivated macrophages; C: Detection of expression of miR-588 by qPCR in DDP-resistant SGC7901 cells co-cultured with M2 macrophages or exosomes from M2 macrophages; D-F: Analysis of cell proliferation by colony formation assay (D), cell apoptosis by flow cytometry (E), and expression of Bax, capase-3, caspase-9, cleaved caspase-3 (c-caspase-3), and cleaved caspase 9 (c-caspase-9) by Western blot analysis (F) in DDP-resistant SGC7901 cells treated with miR-588 inhibitor. Data shown are the mean ± SD, ^bP < 0.01; ^cP < 0.001.