Proteomics identifies a novel role of fibrinogen-like protein 1 in Crohn’s disease

Figure 4 Impact of fibrinogen-like protein 1 on the NF-κB signalling pathway. A and B: Effect of knockdown or overexpression of fibrinogen-like protein 1 (FGL1) on the mRNA expression of FGL1 and NF-κB, as determined by quantitative real-time PCR assay; C: Effect of FGL1 on the protein expression of related proteins in the canonical NF-κB signalling pathway, as determined by Western blot assay. ^aP < 0.01 vs NC; ^bP < 0.01, ^cP < 0.05 vs LPS. NC: Normal control group; LPS: Crohn’s disease (CD) model induced by LPS; LPS+Vector: Transfected with an empty vector based on the CD model; LPS+si-FGL1: Transfected with FGL1 siRNA based on the CD model; LPS+oe-FGL1: Transfected with the FGL1 plasmid based on the CD model; LPS: Lipopolysaccharide; FGL1: Fibrinogen-like protein 1.