Copyright
©The Author(s) 2021.
World J Gastroenterol. Sep 14, 2021; 27(34): 5737-5752
Published online Sep 14, 2021. doi: 10.3748/wjg.v27.i34.5737
Published online Sep 14, 2021. doi: 10.3748/wjg.v27.i34.5737
Table 1 Primer sequences, annealing temperature and product size for methylation-specific polymerase of target genes
| Genes | Forward primer (5’→3’) | Annealing temperature (oC) | Product size (bp) | |
| CDKN2A | M | F: TTATTAGAGGGTGGGGCGGATCGC | 62 | 150 |
| R: GACCCCGAACCGCGACCGTAA | ||||
| U | F: TTATTAGAGGGTGGGGTGGATTGT | 62 | 151 | |
| R: CAACCCCAAACCACAACCATAA | ||||
| MLH1 | M | F: ACGTAGACGTTTTATTAGGGTCGC | 60 | 118 |
| R: CCTCATCGTAACTACCCGCG | ||||
| U | F: TTTTGATGTAGATGTTTTATTAGGGTTGT | 60 | 124 | |
| R: ACCACCTCATCATAACTACCCACA | ||||
| MGMT | M | F: TTTCGACGTTCGTAGGTTTTCGC | 53 | 81 |
| R: GCACTCTTCCGAAAACGAAACG | ||||
| U | F: TTTGTGTTTTGATGTTTGTAGGTTTTTGT | 53 | 93 | |
| R: AACTCCACACTCTTCCAAAAACAAAACA | ||||
- Citation: Lee CC, Kuo YC, Hu JM, Chang PK, Sun CA, Yang T, Li CW, Chen CY, Lin FH, Hsu CH, Chou YC. MTNR1B polymorphisms with CDKN2A and MGMT methylation status are associated with poor prognosis of colorectal cancer in Taiwan. World J Gastroenterol 2021; 27(34): 5737-5752
- URL: https://www.wjgnet.com/1007-9327/full/v27/i34/5737.htm
- DOI: https://dx.doi.org/10.3748/wjg.v27.i34.5737