Estrogen augmented visceral pain and colonic neuron modulation in a double-hit model of prenatal and adult stress

Figure 6 Nerve growth factor expression level in the colon wall. A: Immunohistochemical staining of nerve growth factor (NGF; green) was detected with nuclear counterstaining staining (blue) in controls, chronic adult stress (CAS), chronic prenatal stress (CPS) and CPS + CAS group female rat colon walls. × 400 magnification representative pictures were shown; B: Quantification of NGF levels from colon wall in immunohistochemistry (IHC) (n = 4 rats in each group, one-way ANOVA, ^aP < 0.05 vs control group; ^bP < 0.05 vs CPS group); C: Western blots of NGF protein from control and CPS + CAS female rats colon wall tissue (n = 6 rats in each group, t-test, ^aP < 0.05 vs control group).