Impact of Fusobacterium nucleatum in the gastrointestinal tract on natural killer cells

Figure 2 Experimental design. A: Mice were divided into four goups: Control, anti-control (antibiotics only), phosphate-buffered saline (PBS) (PBS after antibiotics), and Fusobacterium nucleatum (F. nucleatum) (F. nucleatum after antibiotics). The mice were treated with broad-spectrum antibiotics for 3 wk (except control), F. nucleatum was introduced to mice every 2 d for 6 wk by oral gavage; B: After treatments, the levels of total gut bacteria (left) and F. nucleatum (right) were evaluated; C: After administration of F. nucleatum, levels of total bacteria (left) and F. nucleatum (right) in mice were again evaluated. Data are expressed as means ± SD of three independent experiments. PBS: Phosphate-buffered saline.