Y-box binding protein 1 augments sorafenib resistance via the PI3K/Akt signaling pathway in hepatocellular carcinoma

Figure 8 Blocking the PI3K/Akt signaling pathway suppresses the effect of Y-box binding protein 1 on apoptosis. A-D: Apoptosis analysis was conducted by terminal deoxynucleotidyl transferase dUTP nick end labeling staining (A, B) and flow cytometry with Annexin V-APC and PI staining (C, D); E, F: Western blot analysis showed cleaved poly ADP-ribose polymerase (PARP) protein expression. ^aP < 0.05, ^bP < 0.01 vs the indicated groups. Lv: Lentivirus; NC: Negative control; YB-1: Y-box binding protein 1; DAPI: 4’6’-diamidino-2-phenylindole dihydrochloride; TUNEL: Terminal deoxynucleotidyl transferase dUTP nick end labeling; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.