Ubiquitin-specific protease 15 contributes to gastric cancer progression by regulating the Wnt/β-catenin signaling pathway

Figure 5 Lithium chloride partly reverses the effects of ubiquitin-specific protease 15 knockdown on gastric cancer progression. BGC-823 and MKN-45 cells transfected with ubiquitin-specific protease 15 (USP15) small interfering RNA-1 or negative control were incubated with or without lithium chloride (LiCl) (20 mmol/L). A, B: The Cell Counting Kit-8 (CCK-8) assay and colony formation assay evaluated cell proliferation ability; C: Transwell assays evaluated cell invasion ability; D: Western blotting detected the protein expression of β-catenin, c-Myc, and cyclin D1. ^bP < 0.01, data are expressed as the mean ± standard error of the mean.