Ubiquitin-specific protease 15 contributes to gastric cancer progression by regulating the Wnt/β-catenin signaling pathway

Figure 3 Overexpression of ubiquitin-specific protease 15 promotes proliferation, invasion, and epithelial–mesenchymal transition of gastric cancer cells. A: SGC-7901 cells were transfected with ubiquitin-specific protease 15 (USP15) overexpression plasmid, USP15-C269S plasmid (USP15 mutant) or empty vector for 48 h, and the efficiency was detected by Western blotting; B, C: Cell Counting Kit-8 assay and colony formation assay evaluated cell proliferation ability; D, E: Wound healing assay and transwell assay evaluated migration and invasion; F: Western blotting detected epithelial–mesenchymal transition markers (E-cadherin, vimentin, and N-cadherin). ^bP < 0.01, data are expressed as the mean ± standard error of the mean. n.s: Not significant.