Ubiquitin-specific protease 15 contributes to gastric cancer progression by regulating the Wnt/β-catenin signaling pathway

Figure 2 Knockdown of ubiquitin-specific protease 15 inhibits cell proliferation, invasion, and epithelial-mesenchymal transition progression of gastric cancer in vitro. A: BGC-823 and MKN-45 cells were transfected with ubiquitin-specific protease 15 (USP15) small interfering RNA or negative control for 48 h, and the efficiency was detected by Western blotting; B and C: Cell Counting Kit-8 assay and colony formation assay evaluated cell proliferation ability; D and E: Wound healing assay and transwell assays evaluated migration and invasion; F: Western blotting detected epithelial-mesenchymal transition markers (E-cadherin, vimentin, and N-cadherin). ^bP < 0.01, data were expressed as mean ± standard error of the mean.