Human immune repertoire in hepatitis B virus infection

Figure 2 Workflow for immune repertoire sequencing. There are mainly five sections in immune repertoire analysis. 1: Cell/tissue collection. 2: DNA/RNA extraction. The figure illustrates the gene recombination and transcription of T-cell receptors (TCRs) and B-cell receptors (BCRs). The V, D, and J gene segments of TCRs and BCRs undergo somatic rearrangement and gene insertion/deletion before transcription to generate highly variable CDR3 regions, which are then translated as TCRs and immunoglobulins. 3: Library construction by next-generation sequencing methods are used to complete the library preparation and amplification. 4: Data sequencing. 5: Data analysis. BCR: B-cell receptor; CDR3: Complementary determining region 3; TCR: T-cell receptor.