Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells

doi:10.3748/wjg.v27.i24.3581

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 28, 2021; 27(24): 3581-3594
Published online Jun 28, 2021. doi: 10.3748/wjg.v27.i24.3581

Table 1 Primers used in this study

Species	Gene	Primer (5' to 3')
Mouse	β-actin	F: ATCATGTTTGAGACCTTCAACA
Mouse	β-actin	R: CATCTCTTGCTCGAAGTCCA
Mouse	MMP2	F: CTCCTGGTATGAGATAGCAAA
Mouse	MMP2	R: TGCACCACCAACTGCTTAGC
Mouse	MMP9	F: TGCATCTTGGCTTTGCAGCTCTTCCTCATGGC
Mouse	MMP9	R: TGGACCTGTGGGTTGTTGACCTCAAACTTGGC
Mouse	α-SMA	F: CTGACAGAGGCACCACTGAA
Mouse	α-SMA	R: GAAGGAATAGCCACGCTCAG
Human	α-SMA	F: GATCTCAGTGCAGAGGCTCG
Human	α-SMA	R: TTTGCTTGTCCAGGTGGTCC
Human	Ki67	F: TCCTCCAGGGATCCAACGA
Human	Ki67	R: GGCAGGCGGGAGGTCTT
Human	Bcl-2	F: CTTCCCTCATCCTCCTGCTAC
Human	Bcl-2	R: ACAAACTGGGTAAAGGTGATGG
Human	Bax	F: CTCCTGGTATGAGATAGCAAA
Human	Bax	R: TGCACCACCAACTGCTTAGC
Human	Rho	F: TCCTCCAGGGATCCAACGA
Human	Rho	R: GGCAGGCGGGAGGTCTT
Human	β-actin	F: CACTGTGTTGGCGTACAGGT
Human	β-actin	R: TCATCACCATTGGCAATGAG

Note: α-SMA, alpha smooth muscle actin; β-actin, beta actin; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2; MMP, matrix metalloproteinase; Rho, Ras homology family member

Citation: Han QJ, Mu YL, Zhao HJ, Zhao RR, Guo QJ, Su YH, Zhang J. Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells. World J Gastroenterol 2021; 27(24): 3581-3594
URL: https://www.wjgnet.com/1007-9327/full/v27/i24/3581.htm
DOI: https://dx.doi.org/10.3748/wjg.v27.i24.3581