Oncogenic tuftelin 1 as a potential molecular-targeted for inhibiting hepatocellular carcinoma growth

Figure 4 Expression, interfering of tuftelin 1 with proliferation of hepatocellular carcinoma cells. A: Expressions of tuftelin 1 (TUFT1) among different hepatocellular carcinoma (HCC) cell lines were detected by Western blotting, and the expressing levels of all HCC cells were significantly higher than that of the control LO2 cells (^bP < 0.001); A1: The relative ratio from TUFT1 to glyceraldehyde 3-phosphate dehydrogenase with the MHCC-97H cells showed the strongest TUFT1 expression, and the Hep3B cells showed the lowest TUFT1 expression; B: The MHCC-97H cells and interfering with TUFT1 short hairpin RNA (sh-RNA)1-3 (left); the TUFT1 was over-expressed after the Hep3B cells transfected with the constructed pEX-4 (pGCMV/MCS/T2A/EGFP/Neo) plasmid (Right); B1: The TUFT1 expression was significantly inhibited by the TUFT1-shRNA transfection (^bP < 0.001, left); the markedly increasing TUFT1 Level was compared with the control cells (^bP < 0.001, right); C: The TUFT1 activation interfering by TUFT1- shRNA3 significantly inhibited the proliferation of MHCC-97H cells (^aP < 0.05, ^bP < 0.001); D: the over-expression of TUFT1 significantly enhanced the proliferation of the Hep3B cells (^bP < 0.001). Data are presented as mean ± standard error of the mean of at least three independent experiments. NC: Blank control; sh-NC: Negative sh-RNA for tuftelin 1 mRNA.