Partially hydrolyzed guar gum attenuates non-alcoholic fatty liver disease in mice through the gut-liver axis

doi:10.3748/wjg.v27.i18.2160

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May 14, 2021 (publication date) through Nov 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 14, 2021; 27(18): 2160-2176
Published online May 14, 2021. doi: 10.3748/wjg.v27.i18.2160

Table 2 Primer sequences for the targeted mouse genes

Gene	Forward primer	Reverse primer
m_βactin	TATCCACCTTCCAGCAGATGT	AGCTCAGTAACAGTCCGCCTA
m_TNF-α	ATCCGCGACGTGGAACTG	ACCGCCTGGAGTTCTGGAA
m_MCP-1	CTTCCTCCACCACCATGCA	CCAGCCGGCAACTGTGA
m_αSMA	GTCCCAGACATCAGGGAGTAA	TCGGATACTTCAGCGTCAGGA
m_collagen1a1	GCTTCACCTACAGCACCCTTGT	GATGACTGTCTTGCCCCAAGTT
m_Acc	CTGGCTGCATCCATTATGTCA	TGGTAGACTGCCCGTGTGAA
m_Cpt2	GGGCGAGCTTCAGCATATG	GGCCCATCGCTGCTTCTT
m_PPARα	CGATGCTGTCCTCCTTGATGA	CGCGTGTGATAAAGCCATTG
m_PPARγ	CTTGCTGTGGGGATGTCTCA	ATCTCCGCCAACAGCTTCTC
m_TLR4	CCTCTGCCTTCACTACAGAGACTTT	CACAATAACCTTCCGGCTCTTG
m_TLR9	CCTTCGTGGTGTTCGATAAGG	CACCCGCAGCTCGTTATACA

TNF-α: Tumor necrosis factor-α; MCP-1: Monocyte chemotactic protein 1; αSMA: α smooth muscle actin; ACC: Acetyl-CoA carboxylase; CPT2: Carnitine O-palmitoyltransferase II; PPAR: Peroxisome proliferator-activated receptor; TLR: Toll-like receptor.

Citation: Takayama S, Katada K, Takagi T, Iida T, Ueda T, Mizushima K, Higashimura Y, Morita M, Okayama T, Kamada K, Uchiyama K, Handa O, Ishikawa T, Yasukawa Z, Okubo T, Itoh Y, Naito Y. Partially hydrolyzed guar gum attenuates non-alcoholic fatty liver disease in mice through the gut-liver axis. World J Gastroenterol 2021; 27(18): 2160-2176
URL: https://www.wjgnet.com/1007-9327/full/v27/i18/2160.htm
DOI: https://dx.doi.org/10.3748/wjg.v27.i18.2160