Long non-coding RNA TP73-AS1 promotes pancreatic cancer growth and metastasis through miRNA-128-3p/GOLM1 axis

Figure 6 TP73-AS1 increases GOLM1 expression by decreasing miR-128-3p. A: Schematic diagram presenting the putative miR-128-3p recognition site in the GOLM1 sequence; B: Luciferase activity in pancreatic cancer (PC) cells transfected with miR-128-3p and reporter plasmids containing WT or MUT GOLM1 3’UTR; C: The GOLM1 mRNA (up) and protein (down) levels in AsPc-1 and Capan-1 cells with miR-128-3p transfection; D: The GOLM1 mRNA (up) and protein (down) levels in AsPc-1 and Capan-1 cells after TP73-AS1 silencing; E: The expression levels of GOLM1 mRNA in 116 pairs of PC and adjacent non-tumor tissues assessed by quantitative real-time polymerase chain reaction; F: The relationship between miR-128-3p and GOLM1 mRNA levels assessed by Pearson correlation analysis; G: The relationship between TP73-AS1 and GOLM1 mRNA levels assessed by Pearson correlation analysis; H: The protein expression levels of GOLM1 detected by Western blot assay as indicated. ^bP < 0.01. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.