S100 calcium binding protein A6 and associated long noncoding ribonucleic acids as biomarkers in the diagnosis and staging of primary biliary cholangitis

doi:10.3748/wjg.v27.i17.1973

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May 7, 2021 (publication date) through Dec 20, 2024

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World Journal of Gastroenterology

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1007-9327

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World J Gastroenterol. May 7, 2021; 27(17): 1973-1992
Published online May 7, 2021. doi: 10.3748/wjg.v27.i17.1973

Table 1 Primer sequences used in this study

Target gene	Forward sequence (5’→3’)	Reverse sequence (5’→3’)
S100A6	AATGTGCGTTGTGTAAGC	CGGTCCAAGTCTTCCATC
LINC00312	GGAAGGAATACCACAGAAGT	TGAAGAACAGGACATTGACA
LINC00472	AGAGTTGCTGTAGAAGAAGG	AGGAGGAGAGTAGAAGAGAC
LINC01257	TGCTGCGAATGATGACTT	AGGACTTGAATCTGCTACTG
HMGB2	TTACGTTCCTCCCAAAGGTG	TCTTTGGCTGACTGCTCAGA
RC3H2	TTGCAAAGAAATGCGTTGAG	GATTGGCAGACAACTGCTGA
ADAMTS1	CCTCTGTCTGTGTGCAAGGA	GTGGCTCCAGTTGGAATTGT
SERPINE1	CTCTCTCTGCCCTCACCAAC	GTGGAGAGGCTCTTGGTCTG
PALD1	GCCGAAGTTGTTCCCATTTA	GCTGAAAGTCAGAGCCAACC
GSTA4	TCCGTGAGATGGGTTTTAGC	TGCCAAAGAGATTGTGCTTG
ACTA2	TTCAATGTCCCAGCCATGTA	GAAGGAATAGCCACGCTCAG
GAPDH	GCACCGTCAAGGCTGAGAAC	TGGTGAAGACGCCAGTGGA

ACTA2: Actin alpha 2, smooth muscle; ADAMTS1: ADAM metallopeptidase with thrombospondin type 1 motif 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; GSTA4: Glutathione S-transferase alpha 4; HMGB2: High mobility group box 2; PALD1: Phosphatase domain containing paladin 1; RC3H2: Ring finger and CCCH-type domains 2; S100A6: S100 calcium binding protein A6; SERPINE1: Serpin family E member 1.

Citation: Dong XH, Dai D, Yang ZD, Yu XO, Li H, Kang H. S100 calcium binding protein A6 and associated long noncoding ribonucleic acids as biomarkers in the diagnosis and staging of primary biliary cholangitis. World J Gastroenterol 2021; 27(17): 1973-1992
URL: https://www.wjgnet.com/1007-9327/full/v27/i17/1973.htm
DOI: https://dx.doi.org/10.3748/wjg.v27.i17.1973