Abelson interactor 1 splice isoform-L plays an anti-oncogenic role in colorectal carcinoma through interactions with WAVE2 and full-length Abelson interactor 1

Figure 4 Alterations in cellular morphology occur with exogenous Abelson interactor 1 splice isoform-L expression in SW480 colon cancer cells. A: SW480; B: SW480-V; C: SW480-ABI1-SiL3; D: SW480-ABI1-SiL6 cells; E: Cell surface area measured using ImageJ software. The mean values are normalized to those of parental SW480 cells. Data represent the mean ± SD of triplicate experiments. SW480 cells overexpressing Abelson interactor 1 splice isoform-L (ABI1-SiL) showed a 1.4 folds increase in cell surface area, ^bP < 0.01; F: Expression levels of stem-cell and cell differentiation markers determined by quantitative polymerase chain reaction using gene-specific primers. No significant differences were observed between SW480-ABI1-SiL (3 and 6) cells and parental SW480 cells or SW480-V cells (P > 0.05), except for KRT20 in the SW480-ABI1-SiL3 cells (^aP < 0.05).