Impact of preoperative antibiotics and other variables on integrated microbiome-host transcriptomic data generated from colorectal cancer resections

Table 1 Specific Taqman polymerase chain reaction primer and probe sequences for the Fusobacterium nucleatum nusG gene and broad range Taqman primer and probe sequences for the bacterial 16S rRNA gene

Target gene	Sequence
F. nucleatum nusG gene forward primer[29]	5’-CAACCATTACTTTAACTCTACCATGTTCA-3’
F. nucleatum nusG gene reverse primer[29]	5’-GTTGACTTTACAGAAGGAGATTATGTAAAAATC-3’
F. nucleatum nusG gene probe[30]	5’-TCAGCAACTTGTCCTTCTTGATCTTTAAATGAACC-3’
Broad range 16S rRNA gene forward primer[31]	5’-TCCTACGGGAGGCAGCAGT-3’
Broad range 16S rRNA gene reverse primer[31]	5’- GGACTACCAGGGTATCTAATCCTGTT-3’
Broad range 16S rRNA gene probe[31]	5’-CGTATTACCGCGGCTGCTGGCAC-3’

F. nucleatum: Fusobacterium nucleatum.