Huanglian decoction suppresses the growth of hepatocellular carcinoma cells by reducing CCNB1 expression

Figure 7 Huanglian decoction suppressed hepatocellular carcinoma cell growth in vitro by inhibiting the expression level of CCNB1 protein. A and B: Huanglian decoction can reduce the expression level of CCNB1 protein in hepatocellular carcinoma cells, but has no significant effect on the transfected cells; C and D: At 24, 48, 72, and 96 h after transfection, cell proliferation was determined using the MTT assay; E and F: The level of cell apoptosis increased significantly after transfection, and Huanglian decoction had no significant effect on the cells after transfection; G and H: Huanglian decoction can induce G2/M phase arrest of PLC/PRF/5 and HepG2 cells. ^aP < 0.05 vs negative control (NC) group; ^bP < 0.01 vs NC group; ^cP < 0.001 vs NC group. FITC: Fluorescein isothiocyanate.