Long non-coding ribonucleic acid W5 inhibits progression and predicts favorable prognosis in hepatocellular carcinoma

Figure 4 Long non-coding ribonucleic acid W5 inhibits tumor growth in vivo. A: Huh7 cells (5 × 10⁶) stably expressed with long non-coding ribonucleic acid W5 (lncRNA W5) were subcutaneously injected into the left flank of nude mice, and the effect of lncRNA W5 on hepatocellular carcinoma tumor growth was examined every 3 d during the course of the experiment (n = 5); B: A representative image of the xenograft-bearing mice; C: Tumors were isolated from the nude mice after sacrifice. The effects of lncRNA W5 on hepatocellular carcinoma growth were determined by tumor volume and tumor weight; D: LncRNA W5-overexpressing Huh7 cells which stably expressed luciferase were injected into nude mice (n = 5). The bioluminescence photographs of tumor were recorded with the in vivo 200 Imaging System. A representative luciferase signal was recorded from each group at 6 wk after injection. ^bP < 0.01.