lncRNACNN3-206 activates intestinal epithelial cell apoptosis and invasion by sponging miR-212, an implication for Crohn's disease

Figure 7 Effects of lncRNACNN3-206 knockdown on pathological changes in the Crohn’s disease model. A: Results of H&E staining of intestinal mucosal tissue samples from the four groups of mice (a-d magnification, 100 ×; e-f magnification, 400 ×). (a): Few infiltrating neutrophils were observed in the mucosal layer of the intestinal mucosa in the control group. (b): In the TNBS group, the mucosal layer was disrupted, the intestinal crypts were deformed and reduced, and numerous infiltrating neutrophils and mononuclear macrophages were found in the mucosal muscular layer and submucosa, and tissues were observed in the submucosa. (c): In the TNBS + si-lncRNACNN3-206 group, crypt damage was confined to the mucosal layer with a small number of infiltrated neutrophils. (d): In the TNBS + anti-scramble group, some continuous mucosal layers were observed, but the epithelial cells were swollen. A large number of neutrophils and plasma cells were observed in the submucosa and muscularis. (e) and (f): The infiltration of inflammatory cells was observed at higher magnification; B: Distribution of lncRNACNN3-206 in intestinal mucosa of the TNBS group was different from that of the si-lncRNACNN3-206 group (n = 3 for each group). Green fluorescence indicates the probe for lncRNACNN3-206, and blue fluorescence represents cellular structures. The left panels are at 100 × magnification. The right panels are at 400 × magnification; C: Left panels, results of Western blotting for the detection of Caspase10 protein expression in the four groups from two experiments. Right panels, densitometric analysis of Western blotting results. Caspase10 expression was significantly increased in the TNBS and TNBS + anti-scramble groups, but much reduced in the TNBS + si-lncRNACNN3-206 group, in comparison with the TNBS group.